template.json

{
    "Resources": {
        "Ec2Instance": {
            "Type": "AWS::EC2::Instance",
            "Properties": {
                "SecurityGroups": [
                    {
                        "Ref": "InstanceSecurityGroup"
                    },
                    "MyExistingSecurityGroup"
                ],
                "KeyName": "mykey",
                "ImageId": "ami-7a11e213"
            }
        },
        "InstanceSecurityGroup": {
            "Type": "AWS::EC2::SecurityGroup",
            "Properties": {
                "GroupDescription": "Enable SSH access via port 22",
                "SecurityGroupIngress": [
                    {
                        "IpProtocol": "tcp",
                        "FromPort": 22,
                        "ToPort": 22,
                        "CidrIp": "0.0.0.0/0"
                    }
                ]
            }
        }
    }
}