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An error was encountered while running hmmsearch #622

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tgurbich opened this issue Feb 6, 2025 · 0 comments
Open

An error was encountered while running hmmsearch #622

tgurbich opened this issue Feb 6, 2025 · 0 comments
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error Help required for a GTDB-Tk error.

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@tgurbich
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tgurbich commented Feb 6, 2025

Hello! I am running GTDB-Tk on batches of genomes, and I consistently encounter the same error with one specific genome.
Here is the genome that's causing an error: GCA_003150015.1.fna.gz
This is the error:

[2025-02-06 08:44:12] INFO: GTDB-Tk v2.4.0
[2025-02-06 08:44:12] INFO: gtdbtk classify_wf --cpus 1 --pplacer_cpus 1 --genome_dir Genome/ --out_dir GTDB_result -x gz --skip_ani_screen
[2025-02-06 08:44:12] INFO: Using GTDB-Tk reference data version r220: /ref_dbs/release220
[2025-02-06 08:44:12] INFO: Identifying markers in 1 genomes with 1 threads.
[2025-02-06 08:44:12] TASK: Running Prodigal V2.6.3 to identify genes.
                                                                             [2025-02-06 08:44:25] INFO: Completed 1 genome in 12.64 seconds (12.64 seconds/genome).
[2025-02-06 08:44:25] TASK: Identifying TIGRFAM protein families.
==> Processed 0/1 genomes (0%) |               | [?genome/s, ETA ?]Process Process-4:
Traceback (most recent call last):
  File "/usr/local/lib/python3.8/multiprocessing/process.py", line 315, in _bootstrap
    self.run()
  File "/usr/local/lib/python3.8/multiprocessing/process.py", line 108, in run
    self._target(*self._args, **self._kwargs)
  File "/usr/local/lib/python3.8/site-packages/gtdbtk/external/tigrfam_search.py", line 145, in _workerThread
    raise GTDBTkExit(f'Non-zero exit code returned when running hmsearch: {stdout}')
gtdbtk.exceptions.GTDBTkExit: Non-zero exit code returned when running hmsearch: None
[2025-02-06 08:44:25] ERROR: An error was encountered while running hmmsearch.
[2025-02-06 08:44:25] ERROR: Controlled exit resulting from an unrecoverable error or warning.

I am running GTDB-Tk on a slurm cluster using a singularity container (quay.io/biocontainers/gtdbtk:2.4.0--pyhdfd78af_1) and have given the job up to 200 GB of RAM.

I am curious to understand what might be causing this genome to trigger an error.

Thanks a lot in advance!

@tgurbich tgurbich added the error Help required for a GTDB-Tk error. label Feb 6, 2025
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