diff --git a/gui/wxpython/animation/frame.py b/gui/wxpython/animation/frame.py
index 4db0955b942..c6bb476705d 100644
--- a/gui/wxpython/animation/frame.py
+++ b/gui/wxpython/animation/frame.py
@@ -354,9 +354,10 @@ def OnHelp(self, event):
         RunCommand("g.manual", quiet=True, entry="wxGUI.animation")
 
     def OnCloseWindow(self, event):
-        if self.controller.timer.IsRunning():
-            self.controller.timer.Stop()
-        CleanUp(TMP_DIR)()
+        self.close(event)
+
+    def close(self, event=None):
+        self.__del__()  # noqa: PLC2801, C2801
         self._mgr.UnInit()
         self.Destroy()
 
@@ -366,6 +367,7 @@ def __del__(self):
             if self.controller.timer.IsRunning():
                 self.controller.timer.Stop()
         CleanUp(TMP_DIR)()
+        tgis.stop_subprocesses()
 
 
 class AnimationsPanel(wx.Panel):
diff --git a/gui/wxpython/lmgr/frame.py b/gui/wxpython/lmgr/frame.py
index 22714ee01fe..647288edb37 100644
--- a/gui/wxpython/lmgr/frame.py
+++ b/gui/wxpython/lmgr/frame.py
@@ -123,6 +123,9 @@ def __init__(
 
         self._giface = LayerManagerGrassInterface(self)
 
+        # List of wxGUI tools frames with initilized tgis framework suprocesses
+        self.framesWithInitTgisSubprocesses = []
+
         # workspace manager
         self.workspace_manager = WorkspaceManager(lmgr=self, giface=self._giface)
         self._setTitle()
@@ -1698,6 +1701,7 @@ def OnAnimationTool(self, event=None, cmd=None):
         frame = AnimationFrame(parent=self, giface=self._giface)
         frame.CentreOnScreen()
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
         tree = self.GetLayerTree()
         if tree:
@@ -1725,8 +1729,9 @@ def OnTimelineTool(self, event=None, cmd=None):
         except ImportError:
             GError(parent=self, message=_("Unable to start Timeline Tool."))
             return
-        frame = TimelineFrame(None)
+        frame = TimelineFrame(parent=self)
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
     def OnTplotTool(self, event=None, cmd=None):
         """Launch Temporal Plot Tool"""
@@ -1737,6 +1742,7 @@ def OnTplotTool(self, event=None, cmd=None):
             return
         frame = TplotFrame(parent=self, giface=self._giface)
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
     def OnHistogram(self, event):
         """Init histogram display canvas and tools"""
@@ -2281,6 +2287,7 @@ def OnCloseWindowOrExit(self, event):
         if ret != wx.ID_CANCEL:
             self._closeWindow(event)
             if ret == wx.ID_YES:
+                self._stopFramesWithInitTgisSubprocesses()
                 self._quitGRASS()
 
     def _closeWindow(self, event):
@@ -2301,6 +2308,23 @@ def _closeWindow(self, event):
         self._auimgr.UnInit()
         self.Destroy()
 
+    def _stopFramesWithInitTgisSubprocesses(self):
+        """Stop wxGUI tools frames initiallized tgis framework
+        subprocesses
+
+        All these wxGUI Animation, Timeline, Temporal tool initialize tgis
+        framework (messenger and C-interface subprocesses) which require to be
+        properly stopped before quitting GRASS (sending SIGTERM signal), because
+        it causes "freeze" wxGUI tool window and wxGUI processes are not terminated.
+        """
+        try:
+            for frame in self.framesWithInitTgisSubprocesses:
+                if frame:
+                    frame.close()
+        # OSError: handle is closed error
+        except OSError:
+            pass
+
     def _quitGRASS(self):
         """Quit GRASS terminal"""
         shellPid = get_shell_pid()
diff --git a/gui/wxpython/main_window/frame.py b/gui/wxpython/main_window/frame.py
index 84ad713ffa5..f20b31061fb 100644
--- a/gui/wxpython/main_window/frame.py
+++ b/gui/wxpython/main_window/frame.py
@@ -141,6 +141,9 @@ def __init__(
 
         self._giface = LayerManagerGrassInterface(self)
 
+        # List of wxGUI tools frames with initilized tgis framework suprocesses
+        self.framesWithInitTgisSubprocesses = []
+
         # workspace manager
         self.workspace_manager = WorkspaceManager(lmgr=self, giface=self._giface)
         self._setTitle()
@@ -1854,6 +1857,7 @@ def OnAnimationTool(self, event=None, cmd=None):
         frame = AnimationFrame(parent=self, giface=self._giface)
         frame.CentreOnScreen()
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
         tree = self.GetLayerTree()
         if tree:
@@ -1881,8 +1885,9 @@ def OnTimelineTool(self, event=None, cmd=None):
         except ImportError:
             GError(parent=self, message=_("Unable to start Timeline Tool."))
             return
-        frame = TimelineFrame(None)
+        frame = TimelineFrame(parent=self)
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
     def OnTplotTool(self, event=None, cmd=None):
         """Launch Temporal Plot Tool"""
@@ -1893,6 +1898,7 @@ def OnTplotTool(self, event=None, cmd=None):
             return
         frame = TplotFrame(parent=self, giface=self._giface)
         frame.Show()
+        self.framesWithInitTgisSubprocesses.append(frame)
 
     def OnHistogram(self, event):
         """Init histogram display canvas and tools"""
@@ -2390,6 +2396,7 @@ def OnCloseWindowOrExit(self, event):
         if ret != wx.ID_CANCEL:
             self._closeWindow(event)
             if ret == wx.ID_YES:
+                self._stopFramesWithInitTgisSubprocesses()
                 self._quitGRASS()
 
     def _closeWindow(self, event):
@@ -2410,6 +2417,23 @@ def _closeWindow(self, event):
         self._auimgr.UnInit()
         self.Destroy()
 
+    def _stopFramesWithInitTgisSubprocesses(self):
+        """Stop wxGUI tools frames initiallized tgis framework
+        subprocesses
+
+        All these wxGUI Animation, Timeline, Temporal tool initialize tgis
+        framework (messenger and C-interface subprocesses) which require to be
+        properly stopped before quitting GRASS (sending SIGTERM signal), because
+        it causes "freeze" wxGUI tool window and wxGUI processes are not terminated.
+        """
+        try:
+            for frame in self.framesWithInitTgisSubprocesses:
+                if frame:
+                    frame.close()
+        # OSError: handle is closed error
+        except OSError:
+            pass
+
     def _quitGRASS(self):
         """Quit GRASS terminal"""
         shellPid = get_shell_pid()
diff --git a/gui/wxpython/timeline/frame.py b/gui/wxpython/timeline/frame.py
index e1004ce35d4..3dc5b1b41aa 100644
--- a/gui/wxpython/timeline/frame.py
+++ b/gui/wxpython/timeline/frame.py
@@ -88,6 +88,9 @@ def __init__(self, parent, title=_("Timeline Tool")):
         self.Bind(wx.EVT_CLOSE, self.OnClose)
 
     def OnClose(self, event):
+        self.close(event)
+
+    def close(self, event=None):
         """Close the database interface and stop the messenger and C-interface
         subprocesses.
         """
diff --git a/gui/wxpython/tplot/frame.py b/gui/wxpython/tplot/frame.py
index adaed601ebc..9b9fea523f9 100755
--- a/gui/wxpython/tplot/frame.py
+++ b/gui/wxpython/tplot/frame.py
@@ -140,6 +140,9 @@ def __del__(self):
         tgis.stop_subprocesses()
 
     def onClose(self, evt):
+        self.close(evt)
+
+    def close(self, evt=None):
         if self._giface.GetMapDisplay():
             self.coorval.OnClose()
             self.cats.OnClose()