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Hi! I am trying to use this pipeline to scaffold a genome produced with PacBio HiFi technology, but having a high number of contigs (116) with HiC data we generated. If I run the pipeline, however, it finishes without errors and producing the expected output, but the final assembly (out/scaffolds/yahs.out_scaffolds_final.fa) has 117 contigs. How it is possible that after scaffolding with HiC the number of contigs increased? I specify that in my species we should have 30 chromosomes.
In addition, if I try to visualise the hic file (juicebox_input/out_JBAT.hic) in juicebox the file is not loaded. I clicl on "open" but nothing shows.
I guess there may be some problems on yahs? Thanks in advance
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