diff --git a/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/storageengine/dataregion/utils/DiskUsageStatisticUtil.java b/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/storageengine/dataregion/utils/DiskUsageStatisticUtil.java
index 982c6d56aff5b..2f6c6ac816c3a 100644
--- a/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/storageengine/dataregion/utils/DiskUsageStatisticUtil.java
+++ b/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/storageengine/dataregion/utils/DiskUsageStatisticUtil.java
@@ -53,6 +53,7 @@
 public abstract class DiskUsageStatisticUtil implements Closeable {
 
   protected static final Logger logger = LoggerFactory.getLogger(DiskUsageStatisticUtil.class);
+  // Collected files retain a reader reference and its resource read lock until released.
   protected Queue<TsFileResource> resourcesWithReadLock;
   protected final long timePartition;
   protected final Iterator<TsFileResource> iterator;
@@ -100,9 +101,9 @@ protected void acquireReadLocks(List<TsFileResource> resources) {
         if (!resource.isClosed()) {
           continue;
         }
-        resource.readLock();
+        FileReaderManager.getInstance().increaseFileReaderReference(resource, true);
         if (resource.isDeleted() || !resource.isClosed()) {
-          resource.readUnlock();
+          FileReaderManager.getInstance().decreaseFileReaderReference(resource, true);
           continue;
         }
         resourcesWithReadLock.add(resource);
@@ -118,7 +119,7 @@ protected void releaseReadLocks() {
       return;
     }
     for (TsFileResource resource : resourcesWithReadLock) {
-      resource.readUnlock();
+      FileReaderManager.getInstance().decreaseFileReaderReference(resource, true);
     }
     resourcesWithReadLock = null;
   }
@@ -127,10 +128,9 @@ public void calculateNextFile() {
     TsFileResource tsFileResource = iterator.next();
     if (tsFileResource.isDeleted() || calculateWithoutOpenFile(tsFileResource)) {
       iterator.remove();
-      tsFileResource.readUnlock();
+      FileReaderManager.getInstance().decreaseFileReaderReference(tsFileResource, true);
       return;
     }
-    FileReaderManager.getInstance().increaseFileReaderReference(tsFileResource, true);
     try {
       TsFileSequenceReader reader =
           FileReaderManager.getInstance()
@@ -146,7 +146,7 @@ public void calculateNextFile() {
           tsFileResource.getTsFile().getAbsolutePath(),
           e);
     } finally {
-      // this operation including readUnlock
+      // Release the reader reference registered during collection, including its read lock.
       FileReaderManager.getInstance().decreaseFileReaderReference(tsFileResource, true);
       iterator.remove();
     }
diff --git a/iotdb-core/datanode/src/test/java/org/apache/iotdb/db/storageengine/dataregion/utils/TableDiskUsageTest.java b/iotdb-core/datanode/src/test/java/org/apache/iotdb/db/storageengine/dataregion/utils/TableDiskUsageTest.java
index 4f60753fabe6b..230be9289c21e 100644
--- a/iotdb-core/datanode/src/test/java/org/apache/iotdb/db/storageengine/dataregion/utils/TableDiskUsageTest.java
+++ b/iotdb-core/datanode/src/test/java/org/apache/iotdb/db/storageengine/dataregion/utils/TableDiskUsageTest.java
@@ -240,6 +240,14 @@ public void testCalculateTableSizeFromFile() throws Exception {
 
     DataRegionTableSizeQueryContext context = new DataRegionTableSizeQueryContext(true);
     queryTableSize(context);
+    boolean writeLockAcquired = resource1.tryWriteLock();
+    try {
+      Assert.assertTrue("Disk usage statistics should release all read locks", writeLockAcquired);
+    } finally {
+      if (writeLockAcquired) {
+        resource1.writeUnlock();
+      }
+    }
     Assert.assertEquals(1, context.getTimePartitionTableSizeQueryContextMap().size());
     TimePartitionTableSizeQueryContext timePartitionContext =
         context.getTimePartitionTableSizeQueryContextMap().values().iterator().next();