Exporting FCS files of singlet "gate" with compensation values

[MuratDelman]

Dear Dr. Yurtsev,

I work as a cytometrist at the Izmir Institute of Technology. I need to make doublet discrimination and compensation between fluorophores. May I consult you on a few topics on FlowCytometryTools?

Can I perform compensation by using FlowCytometryTools?

I especially need free software or an R/python package in which I can export FCS files of compensated singlet "gate." So, I can use this file for downstream analysis by using R or Python packages.

Can I export FCS files of compensated singlet "gate" by using FlowCytometryTools?

Can I perform dimensionality reduction and clustering for a multicolor panel?

If FlowCytometryTools cannot perform the above-mentioned specifications,

Doublet discrimination, compensation of fluorochromes
Exporting the FCS files of singlet "gate" with all of the fluorochromes’ compensation values
dimensionality reduction and clustering

Could you please recommend me an open source free flow cytometry data analysis software, R package or Python package?

Thank you in advance.

Kind Regards

Murat Delman
[email protected]