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Getting started with the motile plugin | ||
====================================== | ||
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Installation | ||
------------ | ||
This plugin depends on ``ilpy``, which must be installed via conda.:: | ||
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conda create -n motile-plugin python>=3.10 | ||
conda activate motile-plugin | ||
conda install -c conda-forge -c funkelab -c gurobi ilpy | ||
pip install git+https://github.com/funkelab/motile-napari-plugin.git | ||
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If this is successful, you can then run ``napari`` from your command line, and | ||
the motile plugin should be visible in the "Plugins" drop down menu. | ||
Clicking this should open the motile widget on the right of the viewer. | ||
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Input data | ||
---------- | ||
The motile plugin takes as input expects a Labels layer with time as the | ||
first dimension followed by the spatial dimensions (no channels). | ||
You can load an example 2D HeLa Dataset from the `Cell Tracking Challenge`_ | ||
in "Files" -> "Sample Data". | ||
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In the future, we could also support Points layers as input - please react to | ||
`Issue #30`_ if this is important to your use case. While source images are | ||
nice to qualitatively evaluate results, they are not necessary to run tracking. | ||
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Running tracking | ||
---------------- | ||
The motile plugin by default opens to the "Run Editor" view. In this section, | ||
you can pick a name for your run, select an input Labels layer, set | ||
hyperparameters, and start a motile run. Hovering over the title of each | ||
element in the widget will make a tooltip appear describing the purpose | ||
of the element. When you are ready, click the "Run" button to start tracking. | ||
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Viewing run results | ||
------------------- | ||
Clicking the "Run" button will automatically take you to the "Run Viewer". | ||
Here you will see: | ||
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- the solver status | ||
- a graph of the solver gap (an optimization value that should approach zero) | ||
- the hyperparameters of the run | ||
- a button to save the whole run (for persistent viewing in the plugin) | ||
- a button to export just the tracks as a CSV | ||
- a button to return to the Run Editor | ||
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Once the solver completes, you will also see a segmentation and a tracks layer | ||
in the napari viewer. These are the tracking results. | ||
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A list of "Tracking Runs" will also appear at the bottom of the widget. | ||
These are the runs that are stored in memory - if you run tracking multiple | ||
times with different inputs or parameters, you can click back and forth | ||
between the results here. Deleting runs you do not want to keep viewing | ||
is a good idea, since these are stored in memory. Runs that were saved in | ||
previous sessions do not appear here until you load them from disk with the | ||
"Load Run" button. | ||
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.. _Issue #30: https://github.com/funkelab/motile-napari-plugin/issues/30 | ||
.. _Cell Tracking Challenge: https://celltrackingchallenge.net/ |
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.. toctree:: | ||
:maxdepth: 3 | ||
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getting_started | ||
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Indices and tables | ||
================== | ||
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