diff --git a/packages/core/src/js/tracing/onSpanEndUtils.ts b/packages/core/src/js/tracing/onSpanEndUtils.ts
index 45a12a04bb..55dca0b75d 100644
--- a/packages/core/src/js/tracing/onSpanEndUtils.ts
+++ b/packages/core/src/js/tracing/onSpanEndUtils.ts
@@ -14,10 +14,11 @@ const IOS_INACTIVE_CANCEL_DELAY_MS = 5_000;
  * Hooks on span end event to execute a callback when the span ends.
  */
 export function onThisSpanEnd(client: Client, span: Span, callback: (span: Span) => void): void {
-  client.on('spanEnd', (endedSpan: Span) => {
+  const unsubscribe = client.on('spanEnd', (endedSpan: Span) => {
     if (span !== endedSpan) {
       return;
     }
+    unsubscribe();
     callback(endedSpan);
   });
 }
@@ -28,10 +29,11 @@ export const adjustTransactionDuration = (client: Client, span: Span, maxDuratio
     return;
   }
 
-  client.on('spanEnd', (endedSpan: Span) => {
+  const unsubscribe = client.on('spanEnd', (endedSpan: Span) => {
     if (endedSpan !== span) {
       return;
     }
+    unsubscribe();
 
     const endTimestamp = spanToJSON(span).timestamp;
     const startTimestamp = spanToJSON(span).start_timestamp;
@@ -87,10 +89,11 @@ function discardEmptyNavigationSpan(
     return;
   }
 
-  client.on('spanEnd', (endedSpan: Span) => {
+  const unsubscribe = client.on('spanEnd', (endedSpan: Span) => {
     if (endedSpan !== span) {
       return;
     }
+    unsubscribe();
 
     if (!shouldDiscardFn(span)) {
       return;
@@ -164,10 +167,11 @@ export const onlySampleIfChildSpans = (client: Client, span: Span): void => {
     return;
   }
 
-  client.on('spanEnd', (endedSpan: Span) => {
+  const unsubscribe = client.on('spanEnd', (endedSpan: Span) => {
     if (endedSpan !== span) {
       return;
     }
+    unsubscribe();
 
     const children = getSpanDescendants(span);
 
@@ -221,15 +225,18 @@ export const cancelInBackground = (client: Client, span: Span): void => {
     }
   });
 
-  subscription &&
-    client.on('spanEnd', (endedSpan: Span) => {
-      if (endedSpan === span) {
-        debug.log(`Removing AppState listener for ${spanToJSON(span).op} transaction.`);
-        if (inactiveTimeout !== undefined) {
-          clearTimeout(inactiveTimeout);
-          inactiveTimeout = undefined;
-        }
-        subscription?.remove?.();
+  if (subscription) {
+    const unsubscribe = client.on('spanEnd', (endedSpan: Span) => {
+      if (endedSpan !== span) {
+        return;
       }
+      unsubscribe();
+      debug.log(`Removing AppState listener for ${spanToJSON(span).op} transaction.`);
+      if (inactiveTimeout !== undefined) {
+        clearTimeout(inactiveTimeout);
+        inactiveTimeout = undefined;
+      }
+      subscription.remove?.();
     });
+  }
 };
diff --git a/packages/core/test/tracing/onSpanEndUtils.test.ts b/packages/core/test/tracing/onSpanEndUtils.test.ts
new file mode 100644
index 0000000000..fe690c3c70
--- /dev/null
+++ b/packages/core/test/tracing/onSpanEndUtils.test.ts
@@ -0,0 +1,214 @@
+import type { Client, Span } from '@sentry/core';
+import { getClient, startSpanManual } from '@sentry/core';
+import {
+  adjustTransactionDuration,
+  cancelInBackground,
+  ignoreEmptyBackNavigation,
+  ignoreEmptyRouteChangeTransactions,
+  onlySampleIfChildSpans,
+  onThisSpanEnd,
+} from '../../src/js/tracing/onSpanEndUtils';
+import { setupTestClient } from '../mocks/client';
+
+jest.mock('react-native', () => ({
+  AppState: {
+    isAvailable: true,
+    currentState: 'active',
+    addEventListener: jest.fn(() => ({ remove: jest.fn() })),
+  },
+  Platform: { OS: 'android' },
+  NativeModules: { RNSentry: {} },
+}));
+
+/**
+ * Wraps client.on to intercept the unsubscribe functions returned by each call.
+ * Returns a getter for how many times any of those unsubscribe functions were called.
+ */
+function trackUnsubscribes(client: Client): () => number {
+  let count = 0;
+  const originalOn = client.on.bind(client);
+  jest
+    .spyOn(client, 'on')
+    .mockImplementation((hook: Parameters<Client['on']>[0], callback: Parameters<Client['on']>[1]) => {
+      // eslint-disable-next-line @typescript-eslint/no-explicit-any
+      const realUnsubscribe = (originalOn as any)(hook, callback);
+      return () => {
+        count++;
+        realUnsubscribe();
+      };
+    });
+  return () => count;
+}
+
+function createRootSpan(name: string): Span {
+  return startSpanManual({ name, forceTransaction: true }, span => span);
+}
+
+describe('onSpanEndUtils', () => {
+  beforeEach(() => {
+    setupTestClient();
+  });
+
+  afterEach(() => {
+    jest.clearAllMocks();
+  });
+
+  describe('onThisSpanEnd', () => {
+    it('calls callback when the target span ends', () => {
+      const client = getClient()!;
+      const callback = jest.fn();
+      const span = createRootSpan('target');
+
+      onThisSpanEnd(client, span, callback);
+      span.end();
+
+      expect(callback).toHaveBeenCalledTimes(1);
+      expect(callback).toHaveBeenCalledWith(span);
+    });
+
+    it('does not call callback when a different span ends', () => {
+      const client = getClient()!;
+      const callback = jest.fn();
+      const targetSpan = createRootSpan('target');
+      const otherSpan = createRootSpan('other');
+
+      onThisSpanEnd(client, targetSpan, callback);
+      otherSpan.end();
+
+      expect(callback).not.toHaveBeenCalled();
+    });
+
+    it('unsubscribes the listener after the target span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('target');
+
+      onThisSpanEnd(client, span, jest.fn());
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+
+    it('does not call callback for spans ending after the target span', () => {
+      const client = getClient()!;
+      const callback = jest.fn();
+      const targetSpan = createRootSpan('target');
+      const laterSpan = createRootSpan('later');
+
+      onThisSpanEnd(client, targetSpan, callback);
+      targetSpan.end(); // fires callback, listener unsubscribes
+      laterSpan.end(); // listener is gone — callback must not fire again
+
+      expect(callback).toHaveBeenCalledTimes(1);
+    });
+  });
+
+  describe('adjustTransactionDuration', () => {
+    it('unsubscribes the listener after the span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('target');
+
+      adjustTransactionDuration(client, span, 60_000);
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+  });
+
+  describe('ignoreEmptyBackNavigation', () => {
+    it('unsubscribes the listener after the span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('target');
+
+      ignoreEmptyBackNavigation(client, span);
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+  });
+
+  describe('ignoreEmptyRouteChangeTransactions', () => {
+    it('unsubscribes the listener after the span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('Route Change');
+
+      ignoreEmptyRouteChangeTransactions(client, span, 'Route Change', () => true);
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+  });
+
+  describe('onlySampleIfChildSpans', () => {
+    it('unsubscribes the listener after the span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('target');
+
+      onlySampleIfChildSpans(client, span);
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+  });
+
+  describe('cancelInBackground', () => {
+    it('removes the AppState subscription when the span ends normally', () => {
+      const { AppState } = jest.requireMock('react-native');
+      const removeMock = jest.fn();
+      (AppState.addEventListener as jest.Mock).mockReturnValueOnce({ remove: removeMock });
+
+      const client = getClient()!;
+      const span = createRootSpan('target');
+
+      cancelInBackground(client, span);
+      expect(removeMock).not.toHaveBeenCalled();
+
+      span.end();
+      expect(removeMock).toHaveBeenCalledTimes(1);
+    });
+
+    it('unsubscribes the spanEnd listener after the span ends', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const span = createRootSpan('target');
+
+      cancelInBackground(client, span);
+      expect(getUnsubscribeCount()).toBe(0);
+
+      span.end();
+      expect(getUnsubscribeCount()).toBe(1);
+    });
+  });
+
+  describe('listener accumulation', () => {
+    it('does not accumulate listeners across multiple spans', () => {
+      const client = getClient()!;
+      const getUnsubscribeCount = trackUnsubscribes(client);
+      const callbacks = Array.from({ length: 5 }, () => jest.fn());
+
+      for (let i = 0; i < 5; i++) {
+        const span = createRootSpan(`span-${i}`);
+        onThisSpanEnd(client, span, callbacks[i]);
+        span.end();
+      }
+
+      // Every registered listener must have unsubscribed itself
+      expect(getUnsubscribeCount()).toBe(5);
+
+      // Ending a new span must not trigger any of the already-fired callbacks
+      const newSpan = createRootSpan('new');
+      newSpan.end();
+
+      callbacks.forEach(cb => expect(cb).toHaveBeenCalledTimes(1));
+    });
+  });
+});