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krisp_fasta: ingroup sequeneces not conserved #18

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zachary-foster opened this issue May 10, 2023 · 0 comments
Open

krisp_fasta: ingroup sequeneces not conserved #18

zachary-foster opened this issue May 10, 2023 · 0 comments

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@zachary-foster
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It looks like the ingroup sequences are not conserved as I would expect and the phrase in the README 'we want the diagnostic nucleotide to be conserved in our "ingroup" and different in our "outgroup".'
The README also has this output result:

> Alignment 0
CGACAAGATACTCTCGCAGCTTGGTCAG : ingroup0
.........................A.. : ingroup1
.........................G.. : outgroup0;outgroup1;outgroup2
> Alignment 1
TGACGCAGATCATCCCGCGCTTACTGAC : ingroup0
.........................T.. : ingroup1
.........................C.. : outgroup0;outgroup1;outgroup2
=> Found 2 alignments in 0.60 seconds

This does not seem right to me, but maybe I am missing something.
@toopz, Is this result intended or is the "outgroup" the group that the diagnostic being designed for?

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