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examples_new.txt
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all_Stand Sconfocal_Stand Pconfocal_Stand Mphoton_Stand Zstack_multiPoint_timeLapse_Stand emissionWheel_Stand WF_nolaser_Illumination WF_laser_Illumination Sconfocal_laser_Illumination Sconfocal_LED_Illumination Pconfocal_Illumination Mphoton_Illumination All_Wave WF_filterCube_Wave all_Optics mag_Optics transmitted_Optics WF_Detection Sconfocal_Detection Pconfocal_Detection Mphoton_Detection all_Software multiDim_Software multiColor_Software Z_stack_Software timeLapse_Software tile_Software all_Sample live_Sample fixed_Sample FP_Sample dye_Sample transfect_Sample core_Ack
Leica DMI8; Nikon Ti2 Yokogawa CSU-W1 Zeiss LSM 780 Zeiss LSM 780 LUDL MAC6000; Unibliz VMC-D1 "LUDL MAC500, Sutter Lambda 10-3" Excelitas X-Cite 120Q; SOLA SE Light Engine Toptica iChrome MLE Toptica iChrome MLE Excelitas X-Cite 120Q; SOLA SE Light Engine Toptica iChrome MLE Toptica iChrome MLE "Chroma, Catalog# 49002; Semrock LED-DA/FI/TR/Cy5/Cy7 " "Chroma, Catalog# 49002; Semrock LED-DA/FI/TR/Cy5/Cy7 " Olympus; Zeiss "Zeiss 2x Optovar, #431008-9903-000" Phase; DIC Teledyne Photometrics Prime BSI; Andor Zyla 4.2plus; Hamamatsu Flash 4.0 v3 Teledyne Photometrics Prime BSI; Andor Zyla 4.2plus; Hamamatsu Flash 4.0 v3 "PMT, HyD, NDD" "PMT, HyD, NDD" Zen Blue v. 1.0; NIS Elements v. 1.4 "First, a Z-stack was captured, close shutter, then moved to next x,y stage position, open shutter, trigger camera, collect next Z-stack" Images were acquired sequentially; images were acquired in simultaneous channels Nikon Ti Z drive Images were acquired every 30 seconds NIS Optimal path stitching algoritm Nikon universal stage insert; Prior chambered coverglass slide holder H236 Imaged in milliQ water; imaged in ACSF (recipe) "eGFP, tdTomato" JF649 (Janelia Farm 649); MitoTracker Red Promega FuGENE transfection reagent; Thermo Fisher Lipofectamine The authors gratefully acknowledge the MicRoN (Microscopy Resources on the North Quad) Core for their support and assistance in this work.
Compound; Stereo 50 um; 100 um Galvo scanner; Resonant scanner Galvo scanner; Resonant scanner 35 msec Excitation/Emission filter wheel Location in the Lightpath LED; metal halide 405 nm; 488 nm; 561 nm 405 nm; 488 nm; 561 nm 405 nm; 488nm; 647 nm Tunable Lasos Argon laser; WLL2 Tunable MaiTai 405/488/640/670 nm; 470/40m; 480/40x Plan Apo; Plan Fluor 0.45 N.A. CCD; CMOS CCD; CMOS "Multi-alkali, GaASP" "Multi-alkali, GaASP" Author Name; Github or other code repository 20 um range 1 um step size #1.5; #1.0 Nocodozole (Sigma M1404); Phenol Red Thermofisher Prolong Gold Antifade Mountant P10144 50 nM S10 grant: Shared Instrumentation Grant 1S10# funded the instrument in which the data was collected.
253 um unidirectional; bidirectional unidirectional; bidirectional Marzhauser; Prior; ASI (Applied Scientific Instrumentation) in front of the camera; at a camera port; in the infinity space 60 mW; 1W Gas; Solid State Gas; Solid State 10 mW; 1W "Pulsed, pulse width: 2 ps; Continuous Wave (i.e., CW)" "Pulsed, pulse width: 2 ps; Continuous Wave (i.e., CW)" Hard spluttered; soft coated 20X; 10X Analyzer; Polarizer 6.5 x 6.5 um; 13 x 13 um 6.5 x 6.5 um; 13 x 13 um 632 V; 244V 632 V; 244V HCA Fixed JOBS; custom JOBS; ZEN Experiment Designer Poly-L-lysine coated coverslips 1.4 refractive index; 48 hour curing time
Single disk; dual disk 1.58 usec/pixel; 2 usec/pixel 1.58 usec/pixel; 2 usec/pixel Linear encoded; manual stage AOM (Acusto-Optical Modulator); directly modulated AOM (Acusto-Optical Modulator); directly modulated 405 nm; 488nm; 647 nm 760 nm; 1030nm Neutral Density; Notch 0.45 N.A; 1.4 N.A. 512 x 512 pixels; 2048 x 2048 pixels 512 x 512 pixels; 2048 x 2048 pixels -5V; 1% -5V; 1% Shutter was open during acquisition; Shutter closed during acquisition Passive temperature control
500 rpm no averaging; averaging of 4 no averaging; averaging of 4 "0.1 um precision, 25 mm/sec speed" 10 mW; 1W 10 mW; 1W Argon; solid state Argon; solid state 490 nm; 488/640/670 nm Air; oil 2x2 binning 2x2 binning 1.0; 1.6 1.0; 1.6
100 um x 100 um 100 um x 100 um Mad City Labs Nano CZ-500 piezo; Nikon Ti Z drive AOM (Acusto-Optical Modulator); directly modulated AOM (Acusto-Optical Modulator); directly modulated DIC; Phase; IR; UV 12 bit gain 4; 16 bit 12 bit gain 4; 16 bit Operated in Photon Counting Mode Operated in Photon Counting Mode 50% humidity
1024 x1024 pixels; 900 x 900 pixels 1024 x1024 pixels; 900 x 900 pixels "Nosepiece, piezo" 50 mW laser set to 53% transmission Automated dispersion compensation Deep See Zeiss Immersol 518F; Nikon Silicone immersion oil Gain = 100 Gain = 100 Perfused in room temperature ASCF exchanged at a rate of 10 mL per minute
1.58 usec/pixel; 2 usec/pixel 1.58 usec/pixel; 2 usec/pixel 250 um; 500 um Peak output: 1W 10 MHz; 200 MHz 10 MHz; 200 MHz
123 um; 1 A.U. 123 um; 1 A.U. 380 Hz 20%; 2.5 mm/sec 2.125 2.125
0.4 nm 6^-10e 6^-10e
Zeiss Definite Focus; Nikon Perfect Focus System Rolling; Overlap; Global Rolling; Overlap; Global
"Software based transmitted light 2 pass, 100 um range, 150 nm step size progressive algorithm was used"