diff --git a/spring-test/spring-test.gradle b/spring-test/spring-test.gradle
index f703232a89df..7f6401aa4168 100644
--- a/spring-test/spring-test.gradle
+++ b/spring-test/spring-test.gradle
@@ -8,6 +8,7 @@ dependencies {
 	optional(project(":spring-beans"))
 	optional(project(":spring-context"))
 	optional(project(":spring-jdbc"))
+	optional(project(":spring-r2dbc"))
 	optional(project(":spring-orm"))
 	optional(project(":spring-tx"))
 	optional(project(":spring-web"))
@@ -80,6 +81,7 @@ dependencies {
 	testImplementation("org.hibernate.orm:hibernate-core")
 	testImplementation("org.hibernate.validator:hibernate-validator")
 	testImplementation("org.hsqldb:hsqldb")
+	testImplementation("io.r2dbc:r2dbc-h2")
 	testImplementation("org.junit.platform:junit-platform-testkit")
 	testRuntimeOnly("com.sun.xml.bind:jaxb-core")
 	testRuntimeOnly("com.sun.xml.bind:jaxb-impl")
diff --git a/spring-test/src/main/java/org/springframework/test/context/jdbc/R2dbcPopulatorUtils.java b/spring-test/src/main/java/org/springframework/test/context/jdbc/R2dbcPopulatorUtils.java
new file mode 100644
index 000000000000..8d15cae4ec6a
--- /dev/null
+++ b/spring-test/src/main/java/org/springframework/test/context/jdbc/R2dbcPopulatorUtils.java
@@ -0,0 +1,62 @@
+/*
+ * Copyright 2002-2025 the original author or authors.
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+package org.springframework.test.context.jdbc;
+
+import java.util.List;
+
+import io.r2dbc.spi.ConnectionFactory;
+import reactor.core.publisher.Mono;
+
+import org.springframework.core.io.Resource;
+import org.springframework.r2dbc.connection.init.ResourceDatabasePopulator;
+
+/**
+ * R2dbcPopulatorUtils is a separate class to avoid name conflicts with existing
+ * jdbc-related classes.
+ *
+ * <p><b>NOTE:</b> In the current architecture, MergedSqlConfig is implemented
+ * as a package-private method, so it has been placed in
+ * org.springframework.test.context.jdbc.
+ *
+ * @author jonghoon park
+ * @since 7.0
+ * @see SqlScriptsTestExecutionListener
+ * @see MergedSqlConfig
+ */
+public abstract class R2dbcPopulatorUtils {
+
+	static void execute(MergedSqlConfig mergedSqlConfig, ConnectionFactory connectionFactory, List<Resource> scriptResources) {
+		ResourceDatabasePopulator populator = createResourceDatabasePopulator(mergedSqlConfig);
+		populator.setScripts(scriptResources.toArray(new Resource[0]));
+
+		Mono.from(connectionFactory.create())
+				.flatMap(populator::populate)
+				.block();
+	}
+
+	private static ResourceDatabasePopulator createResourceDatabasePopulator(MergedSqlConfig mergedSqlConfig) {
+		ResourceDatabasePopulator populator = new ResourceDatabasePopulator();
+		populator.setSqlScriptEncoding(mergedSqlConfig.getEncoding());
+		populator.setSeparator(mergedSqlConfig.getSeparator());
+		populator.setCommentPrefixes(mergedSqlConfig.getCommentPrefixes());
+		populator.setBlockCommentStartDelimiter(mergedSqlConfig.getBlockCommentStartDelimiter());
+		populator.setBlockCommentEndDelimiter(mergedSqlConfig.getBlockCommentEndDelimiter());
+		populator.setContinueOnError(mergedSqlConfig.getErrorMode() == SqlConfig.ErrorMode.CONTINUE_ON_ERROR);
+		populator.setIgnoreFailedDrops(mergedSqlConfig.getErrorMode() == SqlConfig.ErrorMode.IGNORE_FAILED_DROPS);
+		return populator;
+	}
+}
diff --git a/spring-test/src/main/java/org/springframework/test/context/jdbc/SqlScriptsTestExecutionListener.java b/spring-test/src/main/java/org/springframework/test/context/jdbc/SqlScriptsTestExecutionListener.java
index 5e741cec4a9f..8ee7f17fed84 100644
--- a/spring-test/src/main/java/org/springframework/test/context/jdbc/SqlScriptsTestExecutionListener.java
+++ b/spring-test/src/main/java/org/springframework/test/context/jdbc/SqlScriptsTestExecutionListener.java
@@ -24,6 +24,7 @@
 
 import javax.sql.DataSource;
 
+import io.r2dbc.spi.ConnectionFactory;
 import org.apache.commons.logging.Log;
 import org.apache.commons.logging.LogFactory;
 import org.jspecify.annotations.Nullable;
@@ -45,6 +46,7 @@
 import org.springframework.test.context.jdbc.SqlMergeMode.MergeMode;
 import org.springframework.test.context.support.AbstractTestExecutionListener;
 import org.springframework.test.context.transaction.TestContextTransactionUtils;
+import org.springframework.test.context.transaction.reactive.TestContextReactiveTransactionUtils;
 import org.springframework.test.context.util.TestContextResourceUtils;
 import org.springframework.transaction.PlatformTransactionManager;
 import org.springframework.transaction.TransactionDefinition;
@@ -332,8 +334,13 @@ else if (logger.isDebugEnabled()) {
 			Assert.state(!newTxRequired, () -> String.format("Failed to execute SQL scripts for test context %s: " +
 					"cannot execute SQL scripts using Transaction Mode " +
 					"[%s] without a PlatformTransactionManager.", testContext, TransactionMode.ISOLATED));
-			Assert.state(dataSource != null, () -> String.format("Failed to execute SQL scripts for test context %s: " +
-					"supply at least a DataSource or PlatformTransactionManager.", testContext));
+			if (dataSource == null) {
+				ConnectionFactory connectionFactory = TestContextReactiveTransactionUtils.retrieveConnectionFactory(testContext);
+				Assert.state(connectionFactory != null, () -> String.format("Failed to execute SQL scripts for test context %s: " +
+						"supply at least a DataSource or PlatformTransactionManager or ConnectionFactory.", testContext));
+				R2dbcPopulatorUtils.execute(mergedSqlConfig, connectionFactory, scriptResources);
+				return;
+			}
 			// Execute scripts directly against the DataSource
 			populator.execute(dataSource);
 		}
diff --git a/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/TestContextReactiveTransactionUtils.java b/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/TestContextReactiveTransactionUtils.java
new file mode 100644
index 000000000000..d5b16ae0d4ca
--- /dev/null
+++ b/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/TestContextReactiveTransactionUtils.java
@@ -0,0 +1,109 @@
+/*
+ * Copyright 2002-2025 the original author or authors.
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+package org.springframework.test.context.transaction.reactive;
+
+import java.util.Map;
+
+import io.r2dbc.spi.Connection;
+import io.r2dbc.spi.ConnectionFactory;
+import org.apache.commons.logging.Log;
+import org.apache.commons.logging.LogFactory;
+import org.jspecify.annotations.Nullable;
+
+import org.springframework.beans.BeansException;
+import org.springframework.beans.factory.BeanFactory;
+import org.springframework.beans.factory.BeanFactoryUtils;
+import org.springframework.beans.factory.ListableBeanFactory;
+import org.springframework.test.context.TestContext;
+import org.springframework.transaction.PlatformTransactionManager;
+import org.springframework.util.Assert;
+
+/**
+ * Utility methods for working with transactions and data access related beans
+ * within the <em>Spring TestContext Framework</em>.
+ *
+ * <p>Mainly for internal use within the framework.
+ *
+ * @author jonghoon park
+ * @since 7.0
+ */
+public abstract class TestContextReactiveTransactionUtils {
+
+	/**
+	 * Default bean name for a {@link ConnectionFactory}:
+	 * {@code "connectionFactory"}.
+	 */
+	public static final String DEFAULT_CONNECTION_FACTORY_NAME = "connectionFactory";
+
+
+	private static final Log logger = LogFactory.getLog(TestContextReactiveTransactionUtils.class);
+
+	/**
+	 * Retrieve the {@link ConnectionFactory} to use for the supplied {@linkplain TestContext
+	 * test context}.
+	 * <p>The following algorithm is used to retrieve the {@code ConnectionFactory} from
+	 * the {@link org.springframework.context.ApplicationContext ApplicationContext}
+	 * of the supplied test context:
+	 * <ol>
+	 * <li>Attempt to look up the single {@code ConnectionFactory} by type.
+	 * <li>Attempt to look up the <em>primary</em> {@code ConnectionFactory} by type.
+	 * <li>Attempt to look up the {@code ConnectionFactory} by type and the
+	 * {@linkplain #DEFAULT_CONNECTION_FACTORY_NAME default data source name}.
+	 * </ol>
+	 * @param testContext the test context for which the {@code ConnectionFactory}
+	 * should be retrieved; never {@code null}
+	 * @return the {@code DataSource} to use, or {@code null} if not found
+	 */
+	@Nullable
+	public static ConnectionFactory retrieveConnectionFactory(TestContext testContext) {
+		Assert.notNull(testContext, "TestContext must not be null");
+		BeanFactory bf = testContext.getApplicationContext().getAutowireCapableBeanFactory();
+
+		try {
+			if (bf instanceof ListableBeanFactory lbf) {
+				// Look up single bean by type
+				Map<String, ConnectionFactory> ConnectionFactories =
+						BeanFactoryUtils.beansOfTypeIncludingAncestors(lbf, ConnectionFactory.class);
+				if (ConnectionFactories.size() == 1) {
+					return ConnectionFactories.values().iterator().next();
+				}
+
+				try {
+					// look up single bean by type, with support for 'primary' beans
+					return bf.getBean(ConnectionFactory.class);
+				}
+				catch (BeansException ex) {
+					logBeansException(testContext, ex, PlatformTransactionManager.class);
+				}
+			}
+
+			// look up by type and default name
+			return bf.getBean(DEFAULT_CONNECTION_FACTORY_NAME, ConnectionFactory.class);
+		}
+		catch (BeansException ex) {
+			logBeansException(testContext, ex, Connection.class);
+			return null;
+		}
+	}
+
+	private static void logBeansException(TestContext testContext, BeansException ex, Class<?> beanType) {
+		if (logger.isTraceEnabled()) {
+			logger.trace("Caught exception while retrieving %s for test context %s"
+					.formatted(beanType.getSimpleName(), testContext), ex);
+		}
+	}
+}
diff --git a/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/package-info.java b/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/package-info.java
new file mode 100644
index 000000000000..bbf41088ae04
--- /dev/null
+++ b/spring-test/src/main/java/org/springframework/test/context/transaction/reactive/package-info.java
@@ -0,0 +1,8 @@
+/**
+ * JDBC support classes for the <em>Spring TestContext Framework</em>,
+ * including support for declarative SQL script execution via {@code @Sql}.
+ */
+@NullMarked
+package org.springframework.test.context.transaction.reactive;
+
+import org.jspecify.annotations.NullMarked;
diff --git a/spring-test/src/main/java/org/springframework/test/r2dbc/R2dbcTestUtils.java b/spring-test/src/main/java/org/springframework/test/r2dbc/R2dbcTestUtils.java
new file mode 100644
index 000000000000..1c5beda74524
--- /dev/null
+++ b/spring-test/src/main/java/org/springframework/test/r2dbc/R2dbcTestUtils.java
@@ -0,0 +1,86 @@
+/*
+ * Copyright 2002-2025 the original author or authors.
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+package org.springframework.test.r2dbc;
+
+import java.util.Objects;
+
+import io.r2dbc.spi.ConnectionFactory;
+import org.jspecify.annotations.Nullable;
+import reactor.core.publisher.Mono;
+
+import org.springframework.r2dbc.core.DatabaseClient;
+import org.springframework.util.StringUtils;
+
+/**
+ * {@code R2dbcTestUtils} is a collection of R2DBC related utility functions
+ * intended to simplify standard database testing scenarios.
+ *
+ * @author jonghoon park
+ * @since 7.0
+ * @see org.springframework.r2dbc.core.DatabaseClient
+ */
+public abstract class R2dbcTestUtils {
+
+	/**
+	 * Count the rows in the given table.
+	 * @param connectionFactory the {@link ConnectionFactory} with which to perform R2DBC
+	 * operations
+	 * @param tableName name of the table to count rows in
+	 * @return the number of rows in the table
+	 */
+	public static Mono<Integer> countRowsInTable(ConnectionFactory connectionFactory, String tableName) {
+		return countRowsInTable(DatabaseClient.create(connectionFactory), tableName);
+	}
+
+	/**
+	 * Count the rows in the given table.
+	 * @param databaseClient the {@link DatabaseClient} with which to perform R2DBC
+	 * operations
+	 * @param tableName name of the table to count rows in
+	 * @return the number of rows in the table
+	 */
+	public static Mono<Integer> countRowsInTable(DatabaseClient databaseClient, String tableName) {
+		return countRowsInTableWhere(databaseClient, tableName, null);
+	}
+
+	/**
+	 * Count the rows in the given table, using the provided {@code WHERE} clause.
+	 * <p>If the provided {@code WHERE} clause contains text, it will be prefixed
+	 * with {@code " WHERE "} and then appended to the generated {@code SELECT}
+	 * statement. For example, if the provided table name is {@code "person"} and
+	 * the provided where clause is {@code "name = 'Bob' and age > 25"}, the
+	 * resulting SQL statement to execute will be
+	 * {@code "SELECT COUNT(0) FROM person WHERE name = 'Bob' and age > 25"}.
+	 * @param databaseClient the {@link DatabaseClient} with which to perform JDBC
+	 * operations
+	 * @param tableName the name of the table to count rows in
+	 * @param whereClause the {@code WHERE} clause to append to the query
+	 * @return the number of rows in the table that match the provided
+	 * {@code WHERE} clause
+	 */
+	public static Mono<Integer> countRowsInTableWhere(
+			DatabaseClient databaseClient, String tableName, @Nullable String whereClause) {
+
+		String sql = "SELECT COUNT(0) FROM " + tableName;
+		if (StringUtils.hasText(whereClause)) {
+			sql += " WHERE " + whereClause;
+		}
+		return databaseClient.sql(sql)
+				.map(row -> Objects.requireNonNull(row.get(0, Long.class)).intValue())
+				.one();
+	}
+}
diff --git a/spring-test/src/main/java/org/springframework/test/r2dbc/package-info.java b/spring-test/src/main/java/org/springframework/test/r2dbc/package-info.java
new file mode 100644
index 000000000000..825098dcf7fb
--- /dev/null
+++ b/spring-test/src/main/java/org/springframework/test/r2dbc/package-info.java
@@ -0,0 +1,7 @@
+/**
+ * Support classes for tests based on R2DBC.
+ */
+@NullMarked
+package org.springframework.test.r2dbc;
+
+import org.jspecify.annotations.NullMarked;
diff --git a/spring-test/src/test/java/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.java b/spring-test/src/test/java/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.java
new file mode 100644
index 000000000000..b309e9bac69b
--- /dev/null
+++ b/spring-test/src/test/java/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.java
@@ -0,0 +1,54 @@
+/*
+ * Copyright 2002-2025 the original author or authors.
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+package org.springframework.test.context.aot.samples.r2dbc;
+
+import io.r2dbc.spi.ConnectionFactory;
+import org.junit.jupiter.api.Test;
+import reactor.test.StepVerifier;
+
+import org.springframework.beans.factory.annotation.Autowired;
+import org.springframework.test.annotation.DirtiesContext;
+import org.springframework.test.context.TestPropertySource;
+import org.springframework.test.context.jdbc.Sql;
+import org.springframework.test.context.jdbc.SqlMergeMode;
+import org.springframework.test.context.junit.jupiter.SpringJUnitConfig;
+import org.springframework.test.context.reactive.EmptyReactiveDatabaseConfig;
+
+import static org.assertj.core.api.Assertions.assertThat;
+import static org.springframework.test.context.jdbc.SqlMergeMode.MergeMode.MERGE;
+import static org.springframework.test.r2dbc.R2dbcTestUtils.countRowsInTable;
+
+/**
+ * @author jonghoon park
+ * @since 7.0
+ */
+@SpringJUnitConfig(EmptyReactiveDatabaseConfig.class)
+@SqlMergeMode(MERGE)
+@Sql("/org/springframework/test/context/r2dbc/schema.sql")
+@DirtiesContext
+@TestPropertySource(properties = "test.engine = jupiter")
+public class R2dbcSqlScriptsSpringJupiterTests {
+
+	@Test
+	@Sql // default script --> org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.test.sql
+	void test(@Autowired ConnectionFactory connectionFactory) {
+		StepVerifier.create(countRowsInTable(connectionFactory, "users"))
+				.assertNext(count -> assertThat(count).isEqualTo(1))
+				.verifyComplete();
+	}
+
+}
diff --git a/spring-test/src/test/java/org/springframework/test/context/reactive/EmptyReactiveDatabaseConfig.java b/spring-test/src/test/java/org/springframework/test/context/reactive/EmptyReactiveDatabaseConfig.java
new file mode 100644
index 000000000000..7998d796c918
--- /dev/null
+++ b/spring-test/src/test/java/org/springframework/test/context/reactive/EmptyReactiveDatabaseConfig.java
@@ -0,0 +1,39 @@
+/*
+ * Copyright 2002-2025 the original author or authors.
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *      https://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+package org.springframework.test.context.reactive;
+
+import io.r2dbc.spi.ConnectionFactories;
+import io.r2dbc.spi.ConnectionFactory;
+
+import org.springframework.context.annotation.Bean;
+import org.springframework.context.annotation.Configuration;
+
+/**
+ * Empty reactive database configuration class for SQL script integration tests.
+ *
+ * @author jonghoon park
+ * @since 7.0
+ */
+@Configuration
+public class EmptyReactiveDatabaseConfig {
+
+	@Bean
+	ConnectionFactory connectionFactory() {
+		return ConnectionFactories.get(
+				"r2dbc:h2:mem:///testdb?options=DB_CLOSE_DELAY=-1;DB_CLOSE_ON_EXIT=FALSE");
+	}
+}
diff --git a/spring-test/src/test/resources/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.test.sql b/spring-test/src/test/resources/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.test.sql
new file mode 100644
index 000000000000..8c5bb0587d7a
--- /dev/null
+++ b/spring-test/src/test/resources/org/springframework/test/context/aot/samples/r2dbc/R2dbcSqlScriptsSpringJupiterTests.test.sql
@@ -0,0 +1 @@
+INSERT INTO users VALUES('Daisy');
diff --git a/spring-test/src/test/resources/org/springframework/test/context/r2dbc/schema.sql b/spring-test/src/test/resources/org/springframework/test/context/r2dbc/schema.sql
new file mode 100644
index 000000000000..2068a019d601
--- /dev/null
+++ b/spring-test/src/test/resources/org/springframework/test/context/r2dbc/schema.sql
@@ -0,0 +1,4 @@
+CREATE TABLE users (
+  name VARCHAR(20) NOT NULL,
+  PRIMARY KEY(name)
+);