Since overlap optimization is decoupled form from primer optimization, the script doesn't yet know how to handle situations when it happens that the primer overlap site has duplicates within the sequence, which could in principle be handled via moving the overlap site further inwards to the problematic sequence.
Since overlap optimization is decoupled form from primer optimization, the script doesn't yet know how to handle situations when it happens that the primer overlap site has duplicates within the sequence, which could in principle be handled via moving the overlap site further inwards to the problematic sequence.