BioRoute

BioRoute is a single-file bioinformatics prototype that maps genetic mutation findings onto metabolic pathway disruption models for glycolysis, the Krebs cycle, and the electron transport chain.

Live app: https://bioroute.vercel.app

What It Does

• Visualizes metabolic pathways as horizontal flowcharts.
• Highlights affected enzymatic steps when a tracked gene is searched.
• Parses gene symbols, HGVS-like input, and VCF-like rows.
• Scores deterministic variant-to-pathway impact from effect, zygosity, allele frequency, CADD-style signal, ClinVar-style signal, and pathway criticality.
• Produces pathway burden summaries, predicted metabolite shifts, evidence links, CSV/JSON/text exports, analysis hashes, and local audit trail events.
• Includes a draft regulated-product documentation package under docs/regulatory.

Important Safety Boundary

BioRoute is a research-use prototype. It is not a clinical diagnostic device, is not FDA-cleared or FDA-approved, and does not replace ACMG/AMP variant interpretation or clinician review.

Files

• index.html - complete app, styles, data model, and JavaScript engine.
• work/test_bioroute_dom.js - Node-based smoke test for the pathway UI and scientific engine.
• docs/regulatory/ - draft design-history, risk, verification, validation, privacy, and release-readiness artifacts.

Run Locally

Open index.html in a browser, or serve the directory with any static server.

Test

node work/test_bioroute_dom.js

Expected output:

BioRoute scientific engine smoke test passed

Deployment

The app is static and can be deployed directly to Vercel, GitHub Pages, Netlify, or any static host.