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Restructure biahub #61

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ieivanov
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Restructure repo as discussed in #52

@ieivanov ieivanov linked an issue Mar 17, 2025 that may be closed by this pull request
@ieivanov ieivanov marked this pull request as ready for review March 24, 2025 03:47
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@tayllatheodoro (cc: @ieivanov), please run an integration test with https://github.com/czbiohub-sf/mantis-analysis-template. Please report here if any of the steps don't work as expected.

@@ -13,7 +13,7 @@
from pystackreg import StackReg
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@mattersoflight mattersoflight Mar 26, 2025

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Instead of using StackReg, I suggest implementing or reusing the 3D phase cross correlation that @edyoshikun may have implemented as part of autotracker algorithm. 3D phase cross correlation should work well with bead images. Perhaps you tried and decided that you need to handle XY and Z-registration separately.

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Is ants useful here?

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@mattersoflight mattersoflight left a comment

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@ieivanov having methods and the CLI in the same file does make code quite readable. Most methods have docstrings, but a few do not.

The logic and algorithms for registration and stabilization steps require an iteration. Let's brainstorm tomorrow or Thursday.

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Just ran a very basic deconvolution integration test on this branch, and all worked as expected.

My only minor suggestion is to consider renaming AnalysisSettings.py to analysis_settings.py or just settings.py. Right now AnalysisSettings.py sticks out as the only CamelCase file.

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Restructure biahub
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